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Sampling Liquids from a Deep Container Using a Syringe with an Extension



Personnel           Task
Helper
  • 1a.  Hands two sample jars to collector: 1 plastic, 1 glass
  • 1b.  Opens blunt needle container
  • 1c.  Extends sterile package containing pipette to collector
  • 1d.  Extends open packages containing needle and syringe to collector
Collector
  • 2a.  Loosens lids on sample jars
  • 2a.  Removes syringe from packaging
  • 2c.  Removes blunt needle from packaging and attaches to syringe
Helper
  • 3a.  Opens packaging container extension tubing
  • 3b.  Extends open package to collector
Collector
  • 4a.  Removes extension tubing from sterile package
  • 4b.  Attaches extension to blunt needle
  • 4c.  Measures tubing against container
Helper
  • 5a.  Cuts tubing as indicated by collector
Collector
  • 6a.  Aspirates 5mL to 20mL of the liquid into the pipette
  • 6b.  Removes syringe with needle attached from extension tubing
  • 6c.  Carefully empties 1/2 the syringe into the plastic jar and replaces lid
  • 6d.  Empties the remaining liquid into the glass jar and replaces lid
  • 6e.  Moves to sample decon area with both sample containers
Helper
  • 7a.  Sprays sample containers with disinfectant
Collector
  • 8a.  Checks to ensure containers are completely wetted
  • 8b.  Dries sample containers with clean paper towel
Helper
  • 9a.  Hands parafilm to collector
  • 9b.  Fills out sample information/security labels
Collector
  • 10a.  Applies parafilm to glass container
  • 10b.  Applies information/security labels
Helper
  • 11a.  Opens clear zip bag to be used as secondary containment
Collector
  • 12a.  Places sample container into clear zip bag
Helper
  • 13a.  Closes clear zip bag
  • 13b.  Transports sample through final decon

Return to Sample Collection and Containment Kit page.

Return to Delaware Public Health Laboratory page.



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