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Sampling Liquids From a Surface or Open Container Using a Pipette

Personnel          Task
  • 1a.  Hands two sample jars to collector: 1 plastic, 1 glass
  • 1b.  Opens sterile package containing pipette
  • 1c.  Extends sterile package containing pipette to collector
  • 2a.  Loosens lids on sample jars
  • 2b.  Removes pipette from packaging
  • 2c.  Aspirates 5mL to 20mL of the liquid into the pipette
  • 2d.  Removes syringe with needle attached from extension tubing
  • 2d.  Removes syringe with needle attached from extension tubing
  • 2e.  Carefully empties 1/2 the syringe into the plastic jar and replaces lid
  • 2f.  Empties the remaining liquid into the glass jar and replaces lid
  • 2g.  Moves to sample decon area with both sample containers
  • 3a.  Sprays sample containers with disinfectant
  • 4a.  Checks to ensure containers is completely wetted
  • 4b.  Dries sample containers with clean paper towel
  • 5a.  Hands parafilm to collector
  • 5b.  Fills out sample information/security labels
  • 6a.  Applies parafilm to glass container
  • 6b.  Applies information/security labels
  • 7a.  Opens clear zip bag to be used as secondary containment
  • 8a.  Places sample container into clear zip bag
  • 9a.  Closes clear zip bag
  • 9b.  Transports sample through final decon

Return to Sample Collection and Containment Kit page.

Return to Delaware Public Health Laboratory page.